TFRC–RNA interactions show the regulation of gene expression and alternative splicing associated with IgAN in human renal tubule mesangial cells

Introduction: IgA nephropathy (IgAN) is the most common primary glomerular disease (PGD) which could progress to renal failure and characterized by aberrant immune complex deposition. Transferrin receptor1 (TFRC), an receptor, a potential RNA binding protein (RBP) regulates expression of genes positively associated with cell cycle proliferation involved in IgAN. Molecular mechanisms TFRC affects IgAN development remain unclear. Methods: In this study, was overexpressed human tubular mesangial cells (HRMCs) RNA-sequencing (RNA-seq) improved immunoprecipitation sequencing (iRIP-seq) were performed. The aim identify targets at transcriptional alternative splicing (AS) levels. Results: TFRC-regulated AS enriched mRNA DNA repair, consistent global changes due overexpression (TFRC-OE). Expression potentially IgAN, including CENPH, FOXM1, KIFC1, TOP2A, FABP4, ID1, KIF20A, ATF3, H19, IRF7, H1-2 , AS, CYGB, MCM7 HNRNPH1 investigated RT-qPCR iRIP-seq data analyzed TFRC-bound targets. RCC1 RPPH1 found be proliferation. Discussion: conclusion, molecular identified HRMCs regulate gene transcription AS. considered have as clinical therapeutic target.